The function of Dysbindin, a protein related to schizophrenia, in pancreatic cancer / 中华肝胆外科杂志

ABSTRACT

Objective:To study the effect of dystrobrevin binding protein1 (Dysbindin) in pancreatic cancer and the correlations between Dysbindin and the cholinergic agonist Carbachol.Methods:Pancreatic cancer cell lines with Dysbindin overexpressed or knocked down were constructed by lentivirus based lentivirus or small interference RNA respectively, and the function of Dysbindin and Carbachol on invasion and metastasis of pancreatic cancer cells were investigated by Transwell migration and invasion assay, and the effects of Dysbindin and Carbachol on epithelial mesenchymal transition (EMT) related molecules were detected by Western Blot.Results:When Dysbindin was overexpressed, pancreatic cancer migration and invasion were increasedand compared with control group, expression of E-cadherin was decreased [(0.94±0.00) vs (0.74±0.08)], but N-cadherin [(0.60±0.17) vs (1.33±0.10)] and Snail1 was increased [(0.43±0.11) vs (0.81±0.06)], the differences were statistically significant ( P<0.05), and when Dysbindin was blocked, tumor migration and invasion were inhibited. Carbachol can inhibit the expression of Dysbindin, but increase ZO-1 [(0.16±0.06) vs (1.40±0.15)] and N-cadherin level [(1.34±0.13) vs (1.03±0.14)], the differences were statistically significant ( P<0.05) . Conclusions:Dysbindin can promote metastasis and EMT in pancreatic cancer. And Carbachol can decrease Dysbindin expression and inhibit metastasis and EMT of pancreatic cancer.