Your browser doesn't support javascript.
loading
Mechanism of Salviae Miltiorrhizae Radix et Rhizoma and Carthami Flos Component Compatibility on Inflammatory Factor in Brain Tissue of Rats with Ischemic Stroke / 中国实验方剂学杂志
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 77-83, 2020.
Article Dans Chinois | WPRIM | ID: wpr-872762
ABSTRACT

Objective:

This study intends to study the regulatory effect and mechanism of the effective components of Salviae Miltiorrhizae Radix et Rhizoma and Carthami Flos on inflammatory factors related to cerebral ischemia-reperfusion injury in rats through multiple levels of neuropathologymolecular neurobiology and functional behavior.

Method:

The 32 male rats were randomly divided into four groups: sham group, model group, Danhong components compatibility group(720 mg·kg-1), nimodipine (0.5 mg·kg-1)groups,each group of eight male rats.Cerebral ischemia was established by middle cerebral artery occlusion (MCAO) approach. The treatment was performed immediately and at 6 hour after MCAO.Hematoxylin-eosin (HE)staining was used to check the changes of brain histopathology, immunohistochemistry and Real time polymerase chain reactionReal-time PCR) were used to check the expression of IL-1β and Nrf2 in brain tissueWestern blot was used to detect the protein expression of Nrf2 in brain tissue. The aim is to investigate the treatment mechanism of Salviae Miltiorrhizae Radix et Rhizoma and Carthami Flos components in a rat model of cerebral ischemic-reperfusion injury.

Result:

HE staining results showed, compared with sham group, the surviving neurons amount in the model group was significantly reduced(P<0.01),compared with the MCAO group,the number of surviving neurons in the brain tissue of Salviae Miltiorrhizae Radix et Rhizoma and Carthami Flos component compatibility group and nimodipine group was significantly increased(P<0.05,P<0.01).The results of immunohistochemistry and Real-time PCR showed that,compared with normal group,IL-1β and Nrf2 expression in model group were significantly increased (P<0.01),compared with MCAO group, the expression of IL-1β and Nrf2 in Salviae Miltiorrhizae Radix et Rhizoma and Carthami Flos component compatibility group and the nimodipine group was significantly decreased (P<0.05,P<0.01). Western blot results showed that, compared with sham group, Nrf2 positive expression in model group was much more increased (P<0.01), compared with MCAO group, the expression of Nrf2 in Salviae Miltiorrhizae Radix et Rhizoma and Carthami Flos component compatibility group and the nimodipine group was significantly decreased (P<0.01).

Conclusion:

The combination of effective components of Salviae Miltiorrhizae Radix et Rhizoma and Carthami Flos can significantly down-regulate the expression of IL-1β and Nrf2 proteins.The mechanism is to activate the protein expression of inflammatory pathways, reduce the apoptosis of nerve cells, and finally inhibit the inflammatory response in the process of ischemic stroke injury.

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Type d'étude: Étude pronostique langue: Chinois Texte intégral: Chinese Journal of Experimental Traditional Medical Formulae Année: 2020 Type: Article

Documents relatifs à ce sujet

MEDLINE

...
LILACS

LIS

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Type d'étude: Étude pronostique langue: Chinois Texte intégral: Chinese Journal of Experimental Traditional Medical Formulae Année: 2020 Type: Article