The Antileukemia Activity of ZSTK474 on U937 Cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 17-25, 2021.
Article
Dans Chinois
| WPRIM
| ID: wpr-880025
ABSTRACT
OBJECTIVE@#To investigate the antileukemia activity of phosphatidylinositol-3 kinase (PI3K) inhibitor ZSTK474 on human leukemia cell line U937.@*METHODS@#MTT, soft agar assay, flow cytometric analysis and western blot were used to detect the effect of ZSTK474 on U937 cell proliferation, tumorigenicity, cell cycle, cell apoptosis and phosphorylation levels of the key factor of PI3K/AKT pathway. Chou-Talalay method was used to evaluate the combination of ZSTK474 with Cytarabine or Homoharringtonine.@*RESULTS@#PI3K inhibitor ZSTK474 could inhibit the proliferation and tumorigenicity of U937 cell, induce G@*CONCLUSION@#ZSTK474 can inhibit the pathway of PI3K/AKT, ZSTK474 alone or in combination with Homoharringtonine shows potential antileukemia activity on U937 cells.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Triazines
/
Apoptose
/
Phosphatidylinositol 3-kinases
/
Cellules U937
/
Lignée cellulaire tumorale
/
Prolifération cellulaire
/
Protéines proto-oncogènes c-akt
/
Glycogen synthase kinase 3 beta
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Experimental Hematology
Année:
2021
Type:
Article
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