In silico cloning, expression and bioinformatics analysis of NtODB from common tobacco / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 2836-2844, 2021.
Article
Dans Chinois
| WPRIM
| ID: wpr-887846
ABSTRACT
It has been reported that ODB genes play an important role in homologous recombination-directed DNA repair, suggesting their potential applications in plant breeding. To analyze the expression characteristics of tobacco NtODB gene, the cDNA sequence of NtODB was obtained using in silico cloning technique. The physicochemical properties, signal peptide, and advanced structures of the predicted protein were analyzed using bioinformatics tools. The results showed that the NtODB gene has a 579-bp open reading frame which encodes a protein with 192 amino acid residues. The protein NtODB is predicted to be alkaline and hydrophilic. Real-time quantitative PCR showed that NtODB was constitutively expressed in different tissues. Subcellular localization showed that NtODB was mainly expressed in cell membrane and chloroplast. These results may help us to better understand and elucidate the roles of ODB genes in the homologous recombination-directed DNA repair.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phylogenèse
/
Nicotiana
/
Simulation numérique
/
Séquence nucléotidique
/
Séquence d'acides aminés
/
Clonage moléculaire
/
ADN complémentaire
/
Biologie informatique
/
Amélioration des plantes
Type d'étude:
Étude pronostique
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2021
Type:
Article
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