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HCV core protein promotes liver fibrogenesis via up-regulation of CTGF with TGF-beta1
Experimental & Molecular Medicine ; : 138-145, 2005.
Article Dans Anglais | WPRIM | ID: wpr-90137
ABSTRACT
Liver cirrhosis is one of the major complications of hepatitis C virus (HCV) infection, but the mechanisms underlying HCV-related fibrogenesis are still not clear. Although the roles of HCV core protein remain poorly understood, it is supposed to play an important role in the regulation of cellular growth and hepatocarcinogenesis. The aim of this study was to examine the role of HCV core protein on the hepatic fibrogenesis. We established an in vitro co-culture system with primary hepatic stellate cell (HSC) isolated from rats, and a stable HepG2-HCV core cell line which had been transfected with HCV core gene. The expressions of fibrosis-related molecules transforming growth factor beta1 (TGF-beta1), transforming growth factor b receptor II (TGF beta RII), alpha-smooth muscle actin (alpha-SMA) and connective tissue growth factor (CTGF) were analyzed via histological or molecular methods. In addition, the expression levels of matrix metaloprotinase-2 (MMP-2) and collagen type I (Col I) from the co-cultured media were measured by zymogram and ELISA, respectively. The expressions of alpha-SMA, TGF-beta1, Col I, TGF beta RII and MMP-2 were significantly increased in the co-culture of stable HepG2-HCV core with HSC. Moreover, the significant increases of CTGF and TGF-beta1 in the HCV core-expressing cells were observed by either Northern or Western blot analysis. These results suggest that HCV core protein may contribute to the hepatic fibrogenesis via up-regulation of CTGF and TGF-beta1.
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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Régulation positive / Cellules cultivées / Protéines du core viral / Facteur de croissance transformant bêta / Actines / Rat Sprague-Dawley / Récepteurs TGF-bêta / Protéines précoces immédiates / Techniques de coculture / Matrix metalloproteinase 2 Limites du sujet: Animaux langue: Anglais Texte intégral: Experimental & Molecular Medicine Année: 2005 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Régulation positive / Cellules cultivées / Protéines du core viral / Facteur de croissance transformant bêta / Actines / Rat Sprague-Dawley / Récepteurs TGF-bêta / Protéines précoces immédiates / Techniques de coculture / Matrix metalloproteinase 2 Limites du sujet: Animaux langue: Anglais Texte intégral: Experimental & Molecular Medicine Année: 2005 Type: Article