Expression of Bcl-2 Protein in Ischemia-Reperfused Myocardium of Rabbit / 대한흉부외과학회지
The Korean Journal of Thoracic and Cardiovascular Surgery
; : 924-927, 1998.
Article
de Ko
| WPRIM
| ID: wpr-90399
Bibliothèque responsable:
WPRO
ABSTRACT
BACKGROUND: Myocardial cell death after myocardial infarction or reperfusion is classified into necrosis and apoptosis. Bcl-2 protein is a cytoplasmic protein, which inhibits apoptosis and is expressed in acute stage of myocardial infarction but not in normal heart. This study was performed to investigate whether Bcl-2 protein was expressed respectively to the reperfusion time. MATERIALS AND METHODS: Thirty nine New Zealand white rabbits weighing 1.5-4.8 kg (mean, 2.9kg) were alloted into 7 groups (n=5 in each group) which underwent left anterior descending coronary artery (LAD) occlusion for 30 minutes, followed by reperfusion. The animals were sacrificed at 1, 4, 8, 12, 24 hours, and 3, 7 days after occlusion. Ventricle was excised immediately after intervention. Tissues were fixed in 10% buffured formalin and embedded in paraffin. Bcl-2 protein was detected by immunohistochemical stain with using monoclonal antibody against Bcl-2 protein. RESULTS: The positive immunohistochemical reactivity for Bcl-2 protein was observed in 12, 24 hours, and 3 days reperfusion groups. Bcl-2 protein was detected in salvaged myocytes surrounding the infarcted area. CONCLUSIONS: Bcl-2 protein is expressed at the late acute stage of infarct. Therefore, the expression of Bcl-2 protein may not protect acute cell death, but may play a role in the prevention of late cell death after myocardial is chemia-reperfusion.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Paraffine
/
Reperfusion myocardique
/
Reperfusion
/
Mort cellulaire
/
Apoptose
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Vaisseaux coronaires
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Cytoplasme
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Cellules musculaires
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Formaldéhyde
/
Coeur
Limites du sujet:
Animals
langue:
Ko
Texte intégral:
The Korean Journal of Thoracic and Cardiovascular Surgery
Année:
1998
Type:
Article