Diagnostic value of exocrine LncRNA SPINT1-AS1 in thyroid papillary carcinoma / 中华内分泌外科杂志

ABSTRACT

Objective:To investigate the value of exocrine LncRNA SPINT1-AS1 in diagnosis and prognosis of papillary thyroid carcinoma (PTC) .Methods:qRT-PCR was used to detect the expression of SPINT1-AS1 in PTC tissues, cell lines (TPC-1, BCPAP, K1 and IHH4) , serum exocrine bodies of patients and normal tissues adjacent to cancer, human thyroid normal cell line Nthy-ori 3-1 and healthy volunteers. CCK-8 and colony formation assay were used to detect the effects of SPINT1-AS1 on the proliferation and colony formation of PTC cells. The interaction between SPINT1-AS1 and miR-128-3p, miR-128-3p and ITGA3 was verified by double luciferase report test.Results:The expression of SPINT1-AS1 in PTC tissues and cell lines was significantly higher than that in adjacent normal tissues and Nthy-ori 3-1 cell lines. Knockdown of SPINT1-AS1 inhibited the proliferation and colony formation of PTC cells. Bioinformatics analysis showed that there was a SPINT1-AS1/miR-128-3p/ITGA3 interaction regulatory network in PTC. The direct interaction of SPINT1-AS1, miR-128-3p and ITGA3 was verified by double luciferase reporter test. In addition, SPINT1-AS1 was significantly up-regulated in serum exocrine bodies of patients with PTC.Conclusion:Our study shows that SPINT1-AS1 regulates the miR-128-3p/ITGA3 axis to promote the proliferation and colony formation of PTC cells. Exocrine SPINT1-AS1 may be an effective molecular target for PTC in diagnosis and treatment of PTC.