Anti-inflammatory effect of couplet medicinals of Achyranthes bidentata -Eucommia ulmoides on mouse macro phage RAW264.7 / 中国药房
China Pharmacy
; (12): 308-312, 2022.
Article
Dans Zh
| WPRIM
| ID: wpr-913088
Responsable en Bibliothèque :
WPRO
ABSTRACT
OB JECTIVE To study the anti-inflammatory effect of couplet medicinals of Achyranthes bidentata -Eucommia ulmoides. METHODS Mouse macrophage RAW 264.7 were divided into blank group ,model group ,A. bidentata group(800 μg/mL),E. ulmoides group(800 μg/mL)and low- ,medium- and high- concentration groups of couplet medicinals of A. bidentata - E. ulmoides (400,800,1 600 μg/mL). Excep for blank group and model group ,the other groups were added with corresponding drugs for 6 hours;then blank group was continued to add into the medium ,while model group was added into 10 μg/mL lipopolysaccharide (to induce the inflammatory model );other groups were added into corresponding drugs and 10 μ g/mL lipopolysaccharide. The levels of inflammatory factors [nitric oxide (NO),interleukin-1β(IL-1β),IL-6,tumor necrosis factor-α (TNF-α)] were detected ,and Jin ’s formula was used to evaluate the effects of A. bidentata -E. ulmoides . The expression of inducible nitric oxide synthase (iNOS),cyclooxygenase-2(COX-2),nuclear factor kappa-B (NF-κB)and inhibitor α of NF-κB (IκBα)as well as the phosphorylation of NF-κB p65,IκB kinase(IKK),p38 mitogen-activated protein kinase (p38 MAPK), extracellular signal-regulated kinase (ERK)and c-Jun N-terminal kinase (JNK)were determined. RESULTS Compared with blank group,the level of inflammatory factors ,protein expression of iNOS and COX- 2 as well as the phosphorylation of NF-κB p65, IKK,p38 MAPK,ERK and JNK were increased significantly (P<0.01),while the protein expression of IκBα was decreased significantly(P<0.01). After intervention of couplet medicinals of A. bidentata -E. ulmoides ,the level of inflammatory factors ,the expression or phosphorylation of above proteins were reversed significantly (P<0.05 or P<0.01),and couplet medicinals of A. bidentata-E. ulmoides had a synergistic effect. CONCLUSIONS The couplet medicinals of A. bidentata -E. ulmoides have synergistic anti-inflammatory effect on RAW 264.7 cells. Its mechanism may be related to the inhibition of NF-κB/MAPK signaling pathway related protein expression.
Texte intégral:
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Indice:
WPRIM
Type d'étude:
Prognostic_studies
langue:
Zh
Texte intégral:
China Pharmacy
Année:
2022
Type:
Article