Study of the Expression of E-cadherin, beta-catenin, and c-Met in Gastric Adenocarcinomas

ABSTRACT

PURPOSE: E-cadherin is an adhesion molecule essential for tight connection between cells, forming the cadherin/catenin complex. Truncated beta-catenin disrupts the interaction between E-cadherin and alpha-catenin, leading to the loss of intercellular adhesion. Met protein, the hepatocyte growth factor receptor, plays important roles in signal transduction. We investigated the relationships between the expressions of E-cadherin, beta-catenin, and c-met protein and the clinicopathological and prognostic parameters in gastric adenocarcinomas. MATENRIALS AND METHODS: The patterns of E-cadherin, beta- catenin, and c-met protein expression were studied using immunohistochemistry in formalin-fixed, paraffin-embedded archival tissues from 76 surgically resected gastric adenocarcinomas. RESULTS: Increased expressions of E-cadherin, beta-catenin, and c-met were more significantly correlated in early gastric cancers (EGC) than in advanced gastric cancers (AGC) (P=0.002, P=0.003 and P=0.026). The positive immunoreactivities of all three markers were markedly lower in signet ring-cell type and poorly differentiated type lesions than in intestinal-type lesions. Decreased expression of the beta-catenin protein correlated well with increased tumor invasion depth (P=0.039), and increased lymph node metastasis correlated well with reduced expression of c-met (P=0.046). CONCLUSION: In gastric cancers, reduced expressions of the E-cadherin, beta-catenin, and c-met proteins may play some role in poorer tumor differentiation, deeper tumor invasion, and increased lymph node metastasis. Also, the c-met gene is thought to play a specific role in the mechanism of the yet unknown catenin action.