Group Ⅱ introns and the application in biotechnology: a review / 生物工程学报
Chinese Journal of Biotechnology
; (12): 915-924, 2022.
Article
de Zh
| WPRIM
| ID: wpr-927754
Bibliothèque responsable:
WPRO
ABSTRACT
Group Ⅱ introns are self-splicing ribozymes, which insert directly into target sites in DNA with high frequency through "retrohoming". They specifically and efficiently recognize and splice DNA target sites, endowing themselves with great potential in genetic engineering. This paper reviewed the gene targeting principle of group Ⅱ introns and the application in microbial genetic modification, and then analyzed the limitations of them in multi-functional gene editing and eukaryotes based on the "retrohoming" characteristics and the dependence on high Mg2+ concentration. Finally, we dissected the potential of group Ⅱ introns in the development of novel gene editing tools based on our previous research outcome and the structural characteristics of the introns, hoping to provide a reference for the application of group Ⅱ introns in biotechnology.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
ADN
/
Introns
/
ARN catalytique
/
Ciblage de gène
/
Eucaryotes
langue:
Zh
Texte intégral:
Chinese Journal of Biotechnology
Année:
2022
Type:
Article