Simultaneous determination of content of eight components in Caesalpinia decapetala by UPLC-MS/MS / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 692-700, 2022.
Article
Dans Chinois
| WPRIM
| ID: wpr-927952
ABSTRACT
The present study established the ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) method for simultaneous determination of the content of eight major active components in Caesalpinia decapetala and performed the quality evaluation of C. decapetala from different habitats with the chemical pattern recognition. The analysis was carried out on a Waters BEH C_(18) column(2.1 mm×100 mm, 1.7 μm) at 40 ℃, with the mobile phase of water containing 0.1% formic acid(A) and acetonitrile containing 0.1% formic acid under gradient elution, the flow rate of 0.3 mL·min~(-1), and the injection volume of 1 μL. The electrospray ionization(ESI) source in the negative mode and multiple reaction monitoring(MRM) were used for MS quantitative analysis. The content results were analyzed by the hierarchical cluster analysis(HCA) and principal component analysis(PCA) for the evaluation of the quality difference. Eight components showed good linear relationships within their respective concentration ranges(r>0.999), with the average recoveries of 96.85%-103.4% and RSD of 0.52%-2.8%. The analysis results showed that the quality of samples from different batches was different. The samples were classified into three clusters by HCA and PCA. The method is simple, sensitive, accurate, and efficient, and can be used for the quality evaluation of C. decapetala.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Chromatographie en phase liquide à haute performance
/
Chromatographie en phase liquide
/
Caesalpinia
/
Analyse en composantes principales
/
Spectrométrie de masse en tandem
langue:
Chinois
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2022
Type:
Article
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