The in vivo imaging of zebrafish for chemical vascular toxicity assessment / 中华预防医学杂志
Chinese Journal of Preventive Medicine
; (12): 33-37, 2022.
Article
Dans Zh
| WPRIM
| ID: wpr-935247
Responsable en Bibliothèque :
WPRO
ABSTRACT
Objective: To evaluate the vascular toxicity of chemicals by a real-time observation approach using the transgenic zebrafish. Methods: The spatiotemporal vascular alterations of transgenic zebrafish after chemical exposure were assessed by laser confocal microscopy and high-content screening analysis, respectively. Results: The method using Laser Confocal Microscopy (LCM) is easier to operate and yields high-resolution images, while it is lower throughput and inefficient. In contrast, high-content analysis (HCA) analysis obtains high-quality data of vascular toxicity manifesting whole blood vasculature, whereas it requires delicate operation procedures and advanced experimental conditions. Conclusion: Two kinds of zebrafish imaging methods each have advantages and disadvantages. LCM is suitable for the evaluation of a small number of chemicals. HCA, a cutting-edge technology, has great potential for chemical safety assessment allowing high throughput vascular toxicity tests of a good number of chemicals at a time.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Danio zébré
/
Animal génétiquement modifié
/
Système cardiovasculaire
/
Tests de toxicité
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Chinese Journal of Preventive Medicine
Année:
2022
Type:
Article