Observation of sperm-head vacuoles and sperm morphology under light microscope / 대한생식의학회지
Clinical and Experimental Reproductive Medicine
; : 132-136, 2014.
Article
de En
| WPRIM
| ID: wpr-93555
Bibliothèque responsable:
WPRO
ABSTRACT
OBJECTIVE: The presence of sperm-head vacuoles has been suspected to be deleterious to the outcomes of assisted reproductive technology (ART). It is difficult to accurately distinguish morphologically abnormal sperm with vacuoles under a light microscope. This study was performed to analyze the result of the observation of sperm-head vacuoles using Papanicolaou staining under a light microscope and whether the male partner's age affects these vacuoles. METHODS: Sperm morphology with vacuoles was evaluated using Papanicolaou staining and observed under a light microscope (400x) in 980 men. The normal morphology was divided into three categories (group A, 14% of normal morphology). The criteria for the sperm-head vacuoles were those given in the World Health Organization manual. For the analysis of the age factor, the participants were divided into the following groups: 26-30 years, 31-35 years, 36-40 years, 41-45 years, and 46-50 years. RESULTS: The percentage of sperm-head vacuoles increased with normal sperm morphology (group A vs. groups B, C) (p<0.05). In the case of the age factor, a statistically significant difference was not observed across any of the age groups. CONCLUSION: A majority of the sperm-head vacuoles showed a statistically significant difference among normal morphology groups. Therefore, we should consider the probability of the percentage of sperm-head vacuoles not increasing with age but with abnormal sperm morphology. A further study is required to clarify the effect of the sperm-head vacuoles on ART outcomes.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Spermatozoïdes
/
Vacuoles
/
Organisation mondiale de la santé
/
Facteurs âges
/
Techniques de reproduction assistée
/
Analyse du sperme
Limites du sujet:
Humans
/
Male
langue:
En
Texte intégral:
Clinical and Experimental Reproductive Medicine
Année:
2014
Type:
Article