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Fast determination of bongkrekic acid in plasma by high performance liquid chromatography-tandem mass spectrometry / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases ; (12): 219-221, 2022.
Article Dans Chinois | WPRIM | ID: wpr-935780
ABSTRACT

Objective:

To establish a method for rapid determination of bongkrekic acid (BA) in plasma by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS).

Methods:

In November 2020, plasma samples were extracted by methanol and acetonitrile (1∶1) and purified directly. The samples were separated by C18 column. Gradient elution was carried out with 5 mmol/L ammonium acetate water acetonitrile solution as mobile phase. Under the optimized instrument conditions, the electrospray ionization multiple reaction monitoring (MRM) mode was used, and the external standard method was used for quantitative analysis.

Results:

The linear relationship of BA in plasma was good in the concentration range of 2-100 μg/L, the correlation coefficient was 0.9998, the average recovery was 83.7%-112.0%, the relative standard deviation within and between batches was less than 10%, the detection limit of the method was 0.7 μg/L and the lower limit of quantification was 2.0 μg/L.

Conclusion:

The method is simple, rapid, accurate and sensitive, and can meet the requirements for the determination of BA in blood samples of poisoning patients.
Sujets)

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Acide bongkrékique / Chromatographie en phase liquide à haute performance / Spectrométrie de masse en tandem / Extraction en phase solide Limites du sujet: Humains langue: Chinois Texte intégral: Chinese Journal of Industrial Hygiene and Occupational Diseases Année: 2022 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Acide bongkrékique / Chromatographie en phase liquide à haute performance / Spectrométrie de masse en tandem / Extraction en phase solide Limites du sujet: Humains langue: Chinois Texte intégral: Chinese Journal of Industrial Hygiene and Occupational Diseases Année: 2022 Type: Article