Immune stimulatory activity of BRP-4, an acidic polysaccharide from an edible plant, Basella rubra L
Asian Pacific Journal of Tropical Medicine
;
(12): 849-853, 2014.
Article
Dans Chinois
| WPRIM
| ID: wpr-951787
ABSTRACT
Objective:
To evaluated the immunomodulatory effect of BRP-4, an acidic polysaccharide from Basella rubra (. B. rubra) L on the macrophage activity.Methods:
Phagocytic activity was determined by the ingestion of Latex Beads-Rabbit IgG-FITC using the fluorescent microscopy and flow cytometry analysis and nitric oxide production was measured using Griess reaction assay.Results:
An enhanced production of NO was observed at 10 and 100 μg/mL of BRP-4. The phagocytic activity of macrophage was enhanced in BRP-4 treated RAW264.7 cells. BRP-4 combined with concanavalin A (Con A) provided obvious promotion and strengthening of the proliferation of the splenocytes.Conclusions:
BRP-4, polysaccharide isolated from B. rubra, is suggested to activate macrophage function and stimulate splenocyte proliferation. The strong immunomodulatory activity of BRP-4 confirmed its good potential as an immunotherapeutic adjuvant.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Asian Pacific Journal of Tropical Medicine
Année:
2014
Type:
Article
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