Anti-diabetic effects of Caulerpa lentillifera: Stimulation of insulin secretion in pancreatic ß-cells and enhancement of glucose uptake in adipocytes
Asian Pacific Journal of Tropical Biomedicine
;
(12): 575-580, 2014.
Article
Dans Chinois
| WPRIM
| ID: wpr-951918
ABSTRACT
Objective:
To evaluate anti-diabetic effect of Caulerpa lentillifera (C. lentillifera).Methods:
The inhibitory effect of C. lentillifera extract on dipeptidyl peptidase-IV and α-glucosidase enzyme was measured in a cell free system. Then, interleukin-1ß and interferon-γ induced cell death and insulin secretion were measured in rat insulinoma (RIN) cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and ELISA kit, respectively. Glucose uptake and glucose transporter expression were measured by fluorometry and western blotting, using 3T3-L1 adipocytes.Results:
C. lentillifera extract significantly decreased dipeptidyl peptidase-IV and α-glucosidase enzyme activities, and effectively inhibited cell death and iNOS expression in interleukin-1ß and interferon-γ induced RIN cells. Furthermore, C. lentillifera extract significantly enhanced insulin secretion in RIN cells and glucose transporter expression and glucose uptake in 3T3-L1 adipocytes.Conclusions:
Thus, our results suggest that C. lentillifera could be used as a potential anti-diabetic agent.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Asian Pacific Journal of Tropical Biomedicine
Année:
2014
Type:
Article
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