Genotype analysis of Cryptosporidium spp. prevalent in a rural village in Hwasun-gun, Republic of Korea
The Korean Journal of Parasitology
;
: 27-33, 2006.
Article
Dans Anglais
| WPRIM
| ID: wpr-96036
ABSTRACT
Two species of Cryptosporidium are known to infect man; C. hominis which shows anthroponotic transmission between humans, and C. parvum which shows zoonotic transmission between animals or between animals and man. In this study, we focused on identifying genotypes of Cryptosporidium prevalent among inhabitants and domestic animals (cattle and goats), to elucidate transmittal routes in a known endemic area in Hwasun-gun, Jeollanam-do, Republic of Korea. The existence of Cryptosporidium oocysts was confirmed using a modified Ziehl- Neelsen stain. Human infections were found in 7 (25.9%) of 27 people examined. Cattle cryptosporidiosis cases constituted 7 (41.2%) of 17 examined, and goat cases 3 (42.9%) of 7 examined. Species characterizations were performed on the small subunit of the rRNA gene using both PCR-RFLP and sequence analysis. Most of the human isolates were mixtures of C. hominis and C. parvum genotypes and similar PCR-RFLP patterns were observed in cattle and goat isolates. However, sequence analyses identified only C. hominis in all isolates examined. The natural infection of cattle and goats with C. hominis is a new and unique finding in the present study. It is suggested that human cryptosporidiosis in the studied area is caused by mixtures of C. hominis and C. parvum oocysts originating from both inhabitants and domestic animals.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Polymorphisme de restriction
/
Capra
/
Données de séquences moléculaires
/
Séquence nucléotidique
/
Maladies des bovins
/
Maladies des chèvres
/
Réaction de polymérisation en chaîne
/
Santé en zone rurale
/
Prévalence
/
ADN des protozoaires
Type d'étude:
Étude de prévalence
Limites du sujet:
Animaux
/
Humains
Pays comme sujet:
Asie
langue:
Anglais
Texte intégral:
The Korean Journal of Parasitology
Année:
2006
Type:
Article
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