In vivo determination of the gap2 gene promoter activity in Giardia lamblia
The Korean Journal of Parasitology
;
: 21-26, 2006.
Article
Dans Anglais
| WPRIM
| ID: wpr-96037
ABSTRACT
A shuttle vector for Escherichia coli and Giardia lamblia was modified to produce a reporter plasmid, which monitors the expression of prescribed gene in G. lamblia by measuring its luciferase activity. Promoter regions of the gap2 gene, one of the genes induced during encystation, were cloned into this plasmid, and the resultant constructs were then transfected into trophozoites of G. lamblia. Transgenic trophozoites containing one of the 3 gap2-luc reporters were induced to encystation, and characterized with respect to gap2 gene expression by measuring their luciferase activities. Giardia containing a gap2-luc fusion of 112-bp upstream region showed full induction of luciferase activity during encystation.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Plasmides
/
Facteurs temps
/
Protéines de fusion recombinantes
/
Transfection
/
Génie génétique
/
Expression des gènes
/
Technique de Southern
/
Régions promotrices (génétique)
/
Giardia lamblia
/
Gènes de protozoaire
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
The Korean Journal of Parasitology
Année:
2006
Type:
Article
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