Analysis of chemical components of Shenqi tiaoshen formula / 中国药房

ABSTRACT

OBJECTIVE To analyze chemical components of Shenqi tiaoshen formula （SQTS）. METHODS UPLC-QE-MS method was adopted. The determination was performed on ACQUITY UPLC BEH C18 column with mobile phase consisted of 0.1% formic acid solution-0.1% formic acid acetonitrile solution （gradient elution） at the flow rate of 0.4 mL/min. The column temperature was set at 40 ℃ ， and the sample size was 5 μL. The electrospray ionization source was used to scan positive and negative ions， and the scanning range was m/z 100-1 500. Combined with TCMSP， PubChem and other databases， SQTS active component database was established and the components were identified in combination with relevant literature. RESULTS & CONCLUSIONS Totally 131 chemical components were identified from SQTS， including 23 terpenoids， 22 flavonoids， 21 phenylpropanoids， 12 alkaloids， 11 phenols， 9 amino acid derivatives， 4 fatty acyls， 3 organic acids and others， such as rutin， citrinin， synephrine， cinnamic acid and ginsenoside Rg1，etc. The cracking process of the main components involved the breaking of glycosidic bonds， dehydration， etc.