Delayed allograft rejection by the suppression of class II transactivator
Experimental & Molecular Medicine
; : 210-216, 2006.
Article
de En
| WPRIM
| ID: wpr-96570
Bibliothèque responsable:
WPRO
ABSTRACT
We examined the effect of class II transactivator (CIITA) down-modulation on allograft rejection. To inhibit the function of CIITA, we constructed a series of CIITA mutants and found one exhibiting the dominant-negative effect on the regulation of major histocompatibility complex (MHC) class II expression. To test whether the CIITA dominant-negative mutant reduces immunogenecity, CIITA-transfected melanoma cells were injected into allogeneic host and assessed for immune evading activity against host immune cells. We demonstrated that the CIITA dominant-negative mutant allowed tumor nodules to develop earlier in the lung than control by this tumor challenge study. Furthermore, skin grafts deficient for CIITA also survived longer than wild-type in allogeneic hosts. Both the tumor challenge and skin graft studies suggest the inhibition of CIITA molecules in donor tissue would be beneficial to the control of allo-response.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Transplantation homologue
/
Mélanome expérimental
/
Souris transgéniques
/
Protéines nucléaires
/
Transfection
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Antigènes d'histocompatibilité de classe II
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Activation de la transcription
/
Transactivateurs
/
Interféron gamma
/
Transplantation de peau
Limites du sujet:
Animals
/
Humans
/
Male
langue:
En
Texte intégral:
Experimental & Molecular Medicine
Année:
2006
Type:
Article