Study of the Antischemin’s effect on immune response intensity / Монголын Анагаах Ухаан

ABSTRACT

Background@#The immune response has become the basic use of today’s medical and biological sciences understanding, definition and realization when first introduced into the cognitive level with new theory of nine step closed cycle of protons related to membrane potentials three linear and s-NCM. Medicinal preparations for the immune system derived from traditional herbs have rarely been used therefore, we aim to determine the boost-reduction of immune response with preparation Antischemin with ingredients of Gingko biloba, Astragalus membranaceus and Scutellaria baicalensis.@*Materials and Methods@#This study was carried out on the innovation-research bio-modeling laboratory of the “New Medicine Medical University”. Sheep blood was taken in heparin tube and centrifuged for 10min with speed of 2000rpm and plasma was isolated. Blood components were washed with physiological solution 3-4 times and 10% blood red cell suspension was prepared. Prepared 0.2ml of 10% sheep red cell suspension was injected to mice tail vein to create immune response model (N.K. Jerne and Nordin (1963)). IL-4 (pg), antibody titre SRBC-IgM(ng/mL), and hemagglutinin titre (%) was measured with ELISA kit and compared with control group. Comparing group animals used Salimon 1 ml/kg, Dexamethasone 1 mg/kg orally. The study was conducted in accordance with the approval of Ethics Review Committee of Ministry of Health (November 02, 2018 and approval number 10, №1).@*Result @#In addition, comparing mice which used Antischemin 100 mg/kg orally with sheep blood injected group at day 5, IL-4 level increased by 12.11%, but conclution of antibodies against sheep blood-IgM concentration decreased by 28.7%, and hemagglutinin titre decreased by 27.5%, meaning we observed that the preparation can suppress immune response.@*Сonclusions@#Antischemin preparation meaning observed can suppress immune response.