Effect of ultraviolet C irradiation on expression of transforming growth factor β in wound / 中国康复理论与实践

ABSTRACT

@#ObjectiveTo study the effect of ultraviolet C(UVC) irradiation at different doses on expression of transforming growth factor β(TGF-β) of granulation tissues in wound. MethodsAfter dosing 15mJ/cm2 or 60mJ/cm2 UVC on wound of rats, the expression of TGF-β were observed at both the mRNA level and the protein level by the methods of in situ hybridization and immunohistochemistry respectively. Results At the 7th day after UVC irradiation, the expression of TGF-β in the 15mJ/cm2 group were higher than that in 60mJ/cm2 group and controls (P<0.05)at both the mRNA and the protein level. On the 21st day, the level of TGF-β mRNA in the 60mJ/cm2 group was higher than that in the other two groups(P<0.05).Conclusions At the early stage of wound healing,the treatment of 15mJ/cm2 UVC irradiation promots the expression of TGF-β and might be useful for accelerating wound healing. The level of TGF-β mRNA was up-regulated at the later stage at the dose of 60mJ/cm2 UVC irradiation.