New improved approaches for DYS385 detection / 法医学杂志
Journal of Forensic Medicine
;
(6): 27-29, 2003.
Article
Dans Chinois
| WPRIM
| ID: wpr-982965
ABSTRACT
OBJECTIVE@#To establish more sensitive methods for detection of DYS385 in routine forensic casework.@*METHODS@#The primers recommended by Genome Database (GDB) and Schneider were used to amplify DYS385 respectively. Then, a semi-nested PCR of DYS385 was designed by using the two different primers as outer and inner primer. A series of experiments were carried out to achieve good result by adjusting the ratio of outer/inner primer and optimizing the PCR condition.@*RESULTS@#It showed that an overall 112 bp shorter DYS385 fragments and better electrophoretic separation were obtained by using primer2B. By using the semi-nested PCR approach, the shorter specific DYS385 fragments could be amplified and detectable DNA amounted to 50 pg.@*CONCLUSION@#This method is 20 fold more sensitive than the ordinary method.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Réaction de polymérisation en chaîne
/
Analyse de séquence d'ADN
/
Séquences répétées en tandem
/
Chromosomes Y humains
/
Médecine légale
/
Fréquence d'allèle
Limites du sujet:
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Journal of Forensic Medicine
Année:
2003
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS