Genotype reliability of short tandem repeats typing from minute DNA / 法医学杂志

ABSTRACT

OBJECTIVE@#To understand the forensic practice of DNA profiling from minimal amount of DNA.@*METHODS@#Serial dilutions of DNA were amplified with the PowerPlex 16 System Kit, then the genotyping of short tandem repeat(STR) was performed by ABI 377 DNA automated Sequencer.@*RESULTS@#When the mount of DNA template was less than 250 pg, allelic drop-out apparently occurred at several loci. Other disturbed peaks, such as artefact bands and imbalanced heterozygote, also presented.@*CONCLUSION@#The anomalous results may result in incorrect genotyping. Careful and comprehensive considerations are needed to interpret the STR profile of minute DNA.