Constructing of STR slippage model by optimizing some factors / 法医学杂志

ABSTRACT

OBJECTIVE@#To construct STR slippage model and study factors involved in this procedure.@*METHODS@#DNA samples were amplified with the technology of Degenerate oligonucleotide- primed PCR, then their products were taken as later DNA template and their STR genotype were analyzed by optimizing several factors.@*RESULTS@#STR slippage model was constructed.@*CONCLUSION@#Several factors were involved in the produce of STR slippage, such as amount of modulate DNA, concentration of MgCl2, property of DNA polymerase, motif sequence of STR loci, sample, etc.