A minisequencing technique for genotyping 12 Y-SNP and its genetic polymorphisms / 法医学杂志
Journal of Forensic Medicine
;
(6): 125-129, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-983157
ABSTRACT
OBJECTIVE@#The aim of the present study was to establish a rapid and robust assay used to simultaneously genotype SNPs by the single nucleotide primer extension (minisequencing) with the SNaPshot Kit and obtain the population genetic data in Chinese population in Sichuan. The analysis of single nucleotide polymorphisms (SNPs) is a promising application in forensic casework.@*METHODS@#12 Y-SNPs, which were SRY2627, SRY1532, M13, M20, SRY8299, Tat, M69, M9, 92R7, M17, M19 and M112, were multiple amplificated and the PCR products were pooled, Purified, and then used as templates for the minisequencing reaction with the commol/Lercially available SNaPshot Kit. Then the products of minisequencing reaction were detected by capillary elcetrophoresis.@*RESULTS@#78 genomic DNA individual samples from Sichuan, China and 5 semen stain samples from sexal criminal scene were analyzed and two haplotypes could be identified.@*CONCLUSION@#A rapid method has been established to analyze the 12 Y-SNPs by multiplex PCR and minisequencing. It can be applied in forensic casework successfully.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Infractions sexuelles
/
Os et tissu osseux
/
ADN
/
Séquence nucléotidique
/
Marqueurs génétiques
/
Chine
/
Réaction de polymérisation en chaîne
/
Profilage d'ADN
/
Analyse de séquence d'ADN
/
Amorces ADN
Limites du sujet:
Humains
/
Mâle
Pays comme sujet:
Asie
langue:
Chinois
Texte intégral:
Journal of Forensic Medicine
Année:
2006
Type:
Article
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