Expression of cathepsin-B and -D in rat's brain after traumatic brain injury / 法医学杂志
Journal of Forensic Medicine
;
(6): 404-410, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-983233
ABSTRACT
OBJECTIVE@#To study the expression of cathepsin-B and -D in different time point after traumatic brain injury.@*METHODS@#Traumatic brain injury (TBI) model was established on rats, cathepsin-B and cathepsin-D immunofluorescence staining and confocal microscope analysis were performed. Positive cells were counted by confocal microscope and image analysis techniques were used to determine the morphological changes in each group.@*RESULTS@#Immunofluorescence staining results showed that cathepsin-B was activated 1 hour after TBI while cathepsin-D was not activated until 12hour after TBI. Both of them got to their peak during 4 to 8days, and kept a high level of activating 32days after TBI. Cathepsin-B and -D positive cells did not merge with caspase-3 positive cells until 6 h after TBI.@*CONCLUSION@#Cathepsin-B and -D could be the diagnostic markers of TBI and can estimating time course of lateral TBI. They blocked caspase-3 activation at the beginning period after TBI and started to promote cell death with caspase-3 6 h after TBI.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Facteurs temps
/
Encéphale
/
Lésions encéphaliques
/
Immunohistochimie
/
Cathepsine B
/
Cathepsine D
/
Rat Sprague-Dawley
/
Anatomopathologie légale
/
Modèles animaux de maladie humaine
/
Caspase-3
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Journal of Forensic Medicine
Année:
2006
Type:
Article
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