Haplotype and heteroplasmy in the HV II region of mtDNA in human blood detected by PCR-DGGE / 法医学杂志
Journal of Forensic Medicine
;
(6): 30-32, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-983258
ABSTRACT
OBJECTIVE@#To establish a simple and effective technique for detecting haplotype and heteroplasmy of mtDNA, and investigate their frequencies in Chinese Han population.@*METHODS@#The fragments from 29-290 nt of mtDNA HV II from peripheral leukocytes of 200 unrelated Wuhan Han individuals were analyzed by using PCR-DGGE technique.@*RESULTS@#Seventeen haplotypes were found in the range of 29-290 nt, and the haplotype diversity (HD) was 0.8844. The heterogeneity was observed from 4 individuals, and its frequency was 2%.@*CONCLUSION@#PCR-DGGE is a simple, sensitive and effective technique in analyzing polymorphism and heteroplasmy of mtDNA, and can be used in forensic practice.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Haplotypes
/
ADN mitochondrial
/
Chine
/
Réaction de polymérisation en chaîne
/
Analyse de séquence d'ADN
/
Hétérogénéité génétique
/
Asiatiques
/
Électrophorèse sur gel de polyacrylamide
/
Génétique des populations
/
Mutation
Limites du sujet:
Humains
Pays comme sujet:
Asie
langue:
Chinois
Texte intégral:
Journal of Forensic Medicine
Année:
2007
Type:
Article
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