Detecting ABO blood type of bloodstain with fluorescent antibody method / 法医学杂志

ABSTRACT

UNLABELLED@#OBJECTIVE To explore the advantage and feasibility of fluorescent antibody method for detection of blood type in biological material.@*METHODS@#According to theory of specific binding of antigen and antibody, at first the anti-A monoclonal antibody (MA) and anti-B MA were labeled with the fluorescent, then fluorescent-labeled antibodies (FLA) were bound with corresponding biological material (such as bloodstain) in the optimum condition, finally the ABO blood type of bloodstain was determined under microscope fluorescent.@*RESULTS@#The fluorescent antibody method is highly sensitive, accurate and simple.@*CONCLUSION@#The fluorescent antibody method is an accurate and reliable method for detection of ABO blood type in biological material.