Evaluation of allelic alterations in 13 CODIS STR loci in tumor tissues from human digestive system / 法医学杂志
Journal of Forensic Medicine
;
(6): 280-282, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-983298
ABSTRACT
OBJECTIVE@#To study genetic alterations in 13 CODIS STR loci in various tumor tissue samples from human digestive system.@*METHODS@#Malignant tumor tissues and blood samples taken from 55 different unrelated individuals were collected. DNA samples were extracted using Chelex100 extraction kit, amplified using Profiler and Cofiler PCR amplification kit and analyzed using API 310 analyzer.@*RESULTS@#Aberrant cell divisions were detected in all of the 55 tumor tissue samples, with STR alternations detected in two samples including allelic alteration, partial and complete loss or unbalance of heterozygosity. Moreover, the alternations might occur simultaneously at more than one loci.@*CONCLUSION@#Caution must be taken in STR analysis of tumor tissue samples since the exclusion loci in forensic identification or paternity testing may be resulted from mutations in the tumor tissue.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Tumeurs du pancréas
/
Variation génétique
/
ADN tumoral
/
Réaction de polymérisation en chaîne
/
Perte d'hétérozygotie
/
Séquences répétées en tandem
/
Tumeurs de l'appareil digestif
/
Allèles
/
Tumeurs gastro-intestinales
/
Génotype
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Forensic Medicine
Année:
2007
Type:
Article
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