A study on the time-dependent expression of caspase-6 during repair of skin contusion in rats / 法医学杂志
Journal of Forensic Medicine
;
(6): 325-331, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-983310
ABSTRACT
OBJECTIVE@#To investigate the expression of caspase-6 in rat skin contusion and its surrounding areas during repairment.@*METHODS@#Immunohistochemical SP method and Western blot technique were used to study the expression and activation of caspase-6 in rat skin contusion and its surrounding areas.@*RESULTS@#Weak expression of caspase-6 was detected in cytoplasms of polymorphonuchear cells (PMNs) infiltrated in the injured area at 3 hours post-contusion. The ratio of the caspase-6 positive cells was low (25.78 +/- 1.38)%. The expression of caspase-6 was increased prominently (47.70 +/- 5.14)% at 12 hours post-contusion. Almost all of the PMNs, mononuclear cells (MNCs) and fibroblastic cells (FBCs) were caspase-6 positive with both cytoplasm and nucleus staining (54.58 +/- 5.64)% on post-contusion day 3. The expression of caspase-6 decreased gradually thereafter. The expression of the 34-kDa pro-caspase-6 was detected by Western blot in both control and the post-contusion groups with time dependent dynamics.@*CONCLUSION@#These results suggest that caspase-6 may play a major role in trauma-induced inflammatory response. Since caspase-6 shows a timely dependent expression in PMNs, MNCs and FBCs during skin injury repair in rat, it may be used as a marker for the contusion age determination,
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Peau
/
Facteurs temps
/
Cicatrisation de plaie
/
Immunohistochimie
/
Monocytes
/
Technique de Western
/
Rat Sprague-Dawley
/
Apoptose
/
Contusions
/
Caspase-6
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Journal of Forensic Medicine
Année:
2007
Type:
Article
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