Age-dependent mitochondrial DNA 4977bp depletion in human skeletal muscle / 法医学杂志
Journal of Forensic Medicine
;
(6): 438-440, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-983335
ABSTRACT
OBJECTIVE@#To explore depletion of human mitochondrial DNA 4977-bp and its relation with aging.@*METHODS@#Total DNA (nuclear and mtDNA) was extracted from 100mg muscle tissue. UV light illumination of ethidium bromide-stained PCR products was used to study the depletion of mtDNA (wild-type or mutant).@*RESULTS@#The proportions of mtDNA depletion in human skeletal muscle could be determined. The frequency of mtDNA 4977-bp depletion in different age groups (0-9, 10-19, 20-29, 30-39, 40-49, 50-59, 60-69, 70-79, 80-89, 90-99) was 0%, 0%, 0.003%, 0.011%, 0.015%, 0.033%, 0.038%, 0.062%, 0.069%, and 0.091%, respectively.@*CONCLUSION@#Our findings suggest that the frequency of the mtDNA4977 depletion in human skeletal muscle increases with age. It might be useful for human age estimation.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Vieillissement
/
ADN mitochondrial
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Réaction de polymérisation en chaîne
/
Facteurs âges
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Délétion de séquence
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Amorces ADN
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Muscles squelettiques
/
Mitochondries du muscle
Limites du sujet:
Adolescent
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Adulte
/
Adulte très âgé
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Aged80
/
Enfant
/
Enfant d'âge préscolaire
/
Femelle
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Humains
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Bébé
/
Mâle
langue:
Chinois
Texte intégral:
Journal of Forensic Medicine
Année:
2007
Type:
Article
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