Determination of ethyl glucuronide in blood by GC-MS/MS / 法医学杂志
Journal of Forensic Medicine
;
(6): 431-433, 2009.
Article
Dans Chinois
| WPRIM
| ID: wpr-983519
ABSTRACT
OBJECTIVE@#To develop a method for determining ethyl glucuronide(EtG) in human blood with gas chromatograph-tandem mass spectrometry (GC-MS/MS).@*METHODS@#Human blood protein was precipitated with acetonitrile. The supernatant was transferred and air flow dried after centrifugated. The residue was derived with N, O-bis (trimethylsilyl)trifluoroacetamide (BSTFA), and analyzed with GC-MS/MS.@*RESULTS@#The detection limit of EtG in blood was 0.05 microg/mL. Calibration curve covered a span from 0.1-10 microg/mL with a good linear relationship (r = 0.999 9). The method showed a excellent performance when was used to authentic blood sample analysis for EtG.@*CONCLUSION@#The method is suitable for blood EtG analysis.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Consommation d'alcool
/
Marqueurs biologiques
/
Reproductibilité des résultats
/
Sensibilité et spécificité
/
Alcoolisme
/
Toxicologie médicolégale
/
Glucuronates
/
Chromatographie gazeuse-spectrométrie de masse
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Forensic Medicine
Année:
2009
Type:
Article
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