DNA extraction from decomposed tissue by double-digest and magnetic beads methods / 法医学杂志

ABSTRACT

OBJECTIVE@#To study the effect of the double-digest and magnetic beads method for DNA extraction from 3 types of decomposed tissues.@*METHODS@#DNA of cartilages, nails and joint capsule in 91 highly decomposed corpses which had not been extracted by common magnetic beads method, were prepared with the double-digest and magnetic beads methods, and quantified with Quantifiler kit, followed by amplification with Sinofiler kit or Minifiler kit.@*RESULTS@#DNA concentration extracted from the 91 highly decomposed cartilages, nails and joint capsule samples was 0-0.225 ng/microL. Sixty-two samples whose DNA concentration were more than 0.020 ng/microL had obtained 9 or more STR loci successfully. The detection rate was 68.13%.@*CONCLUSION@#The successful rate of STR genotyping for the 3 types of decomposed tissues can be significantly improved by the double-digest and magnetic beads methods.