Time-dependent expression of vascular endothelial growth factor after acute myocardial ischemia in rats / 法医学杂志
Journal of Forensic Medicine
;
(6): 179-184, 2012.
Article
Dans Chinois
| WPRIM
| ID: wpr-983732
ABSTRACT
OBJECTIVE@#To investigate the immunohistochemical distributions and expressions of vascular endothelial growth factor (VEGF) in the model of rat myocardial ischemia.@*METHODS@#The model of myocardial ischemia was established by ligating the left anterior descending (LAD) coronary artery of rats. The changes of VEGF expression were detected by immunohistochemistry and Western blot at time points after myocardial ischemia. The electrocardiographic changes were evaluated uninterruptedly.@*RESULTS@#The expression of VEGF was not be found in control group. Fifteen minutes after LAD ligation, weak positive expression of VEGF were found in the ischemic myocardium. The expression of VEGF reached the peak at 3 hours after ligation. The VEGF distribution was mainly localized in the ischemic and peri-ischemic regions. Six hours after LAD ligation, the expression of VEGF decreased comparing with 3 hours and showed a relatively higher level. Fatal arrhythmia was found in nine rats by the electrocardiograph.@*CONCLUSION@#The immunohistochemical staining of VEGF could be helpful for investigating the location and severity of acute myocardial ischemia. Fatal arrhythmia may be secondary to myocardial ischemia.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Facteurs temps
/
Immunohistochimie
/
Technique de Western
/
Maladie aigüe
/
Tachycardie ventriculaire
/
Rat Sprague-Dawley
/
Ischémie myocardique
/
Myocytes cardiaques
/
Facteur de croissance endothéliale vasculaire de type A
/
Anatomopathologie légale
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Journal of Forensic Medicine
Année:
2012
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS