Identification of sibling brothers using STR and Y-biallelic markers / 法医学杂志
Journal of Forensic Medicine
;
(6): 190-194, 2012.
Article
Dans Chinois
| WPRIM
| ID: wpr-983735
ABSTRACT
OBJECTIVE@#To explore the methods for identification of sibling brothers with Y-STR locus mutation by detection of genetic markers on autosome and Y-biallelic.@*METHODS@#Goldeneye 20A and 18NC kit were used to genotyped the 35 STRs on autosome from two men. PowerPlex Y kit and Yfiler kit were used to genotyped the 16 STRs on Y chromosome full sibling index was calculated by ITO method. Twenty Y-biallelic markers were genotyped by fragment length discrepant allele specific PCR or general PCR.@*RESULTS@#Relationship of sibling brothers was found to have mutation of 2 loci on 16 Y-STR and the identical genetype of 20 Y-biallelic markers as well as a cumulative full sibling index of 4.3149 x 10(6) from 35 STRs on autosome.@*CONCLUSION@#In identification of paternal linage of Y-STR mutation, more genetic information can be acquired by detection of Y-biallelic markers including SNP and InDel.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Polymorphisme génétique
/
Marqueurs génétiques
/
Réaction de polymérisation en chaîne
/
Répétitions microsatellites
/
Chromosomes Y humains
/
Fratrie
/
Allèles
/
Génétique légale
/
Locus génétiques
/
Fréquence d'allèle
Limites du sujet:
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Journal of Forensic Medicine
Année:
2012
Type:
Article
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