Method development and validation for determining 1,3-butadiene in human blood by gas chromatography-mass spectrometry and head-space gas chromatography / 法医学杂志
Journal of Forensic Medicine
;
(6): 110-115, 2013.
Article
Dans Anglais
| WPRIM
| ID: wpr-983802
ABSTRACT
To develop a simple, validated method for identifying and quantifying 1,3-butadiene (BD) in human blood by gas chromatography-mass spectrometry (GC-MS) and head-space gas chromatography (HS-GC). BD was identified by GC-MS and HS-GC, and quantified by HS-GC. The method showed that BD had a good linearity from 50 to 500 microg/mL (r > 0.99). The limits of detection and quantification were 10 microg/mL and 50 microg/mL, respectively. Both the intra-day precision and inter-day precision were < 6.08%, and the accuracy was 96.98%-103.81%. The method was applied to an actual case, and the concentration of BD in the case was 242 microg/mL in human blood. This simple method is found to be useful for the routine forensic analysis of acute exposure to BD.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Solvants
/
Température
/
Butadiènes
/
Reproductibilité des résultats
/
Sensibilité et spécificité
/
Toxicologie médicolégale
/
Intoxication au gaz
/
Chromatographie gazeuse-spectrométrie de masse
Limites du sujet:
Adulte
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
Journal of Forensic Medicine
Année:
2013
Type:
Article
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