Nitric oxide mediated TNF-α, IL-1β gene expression in liver induced by crush injury of rat's soft tissues / 法医学杂志
Journal of Forensic Medicine
;
(6): 250-256, 2014.
Article
Dans Chinois
| WPRIM
| ID: wpr-983911
ABSTRACT
OBJECTIVE@#To explore the effect of nitric oxide (NO) on the gene expression of hepatic TNF-α and IL-1β by crush injury of rat's soft tissues.@*METHODS@#Rats were randomly divided into sham group, crush group, crush+aminoguanidine (AG) group, and crush+L-arginine (L-Arg) group. Activities of ALT and AST as well as NO level in serum were measured. Gene expressions of TNF-α and IL-1β were detected with RT-PCR.@*RESULTS@#Obvious increase in TNF-α and IL-1β mRNA expression was detected in the crush group compared with the sham group (P<0.05). After pretreated L-Arg, expressions of TNF-α and IL-1β mRNA were markedly increased (P<0.05). After pretreated AG, those indices obviously decreased (P<0.05). Activities of ALT and AST enhanced and NO level increased in the crush group compared with the sham group (P<0.05). Pretreatment with L-Arg or AG led to substantial increased or reduced activities of ALT and AST as well as NO levels, respectively.@*CONCLUSION@#Endogenous NO mediated TNF-α, IL-1β mRNA up expression in liver induced by increased production of NO after crush injury of rat's soft tissues.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Plaies et blessures
/
ARN messager
/
Expression des gènes
/
Facteur de nécrose tumorale alpha
/
Interleukine-1 bêta
/
Foie
/
Monoxyde d'azote
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Journal of Forensic Medicine
Année:
2014
Type:
Article
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