Simultaneous screening for 45 poisonous alkaloids in blood by LC-MS/MS / 法医学杂志
Journal of Forensic Medicine
;
(6): 28-33, 2015.
Article
Dans Anglais
| WPRIM
| ID: wpr-983961
ABSTRACT
OBJECTIVE@#To develop a liquid chromatography-tandem mass spectrometry (LC-MS/MS) screening method for 45 poisonous alkaloids in blood.@*METHODS@#Identification was based on the compound's retention time and two precursor-to-production transitions. The method involved a liquid-liquid extraction (LLE) followed by LC-MS/MS with multiple-reaction monitoring (MRM). When 1 mL of blood was extracted with diethyl ether at pH = 9.2 with SKF525A as the internal standard, the target compounds were analyzed with LC-MS/MS in the positive ionization mode.@*RESULTS@#The target alkaloids had good linearity (r>0.995 1), both the intra-day precision and inter-day precision being less than 14.77%. The limits of detection ranged from 0.05 to 25 ng/mL in blood.@*CONCLUSION@#The method is selective and sensitive in detecting poisonous alkaloids with a total running time of 12 minutes; therefore it was successfully applied to some actual cases of suspected alkaloids poisoning.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Spectrométrie de masse
/
Chromatographie en phase liquide
/
Sensibilité et spécificité
/
Alcaloïdes
/
Spectrométrie de masse en tandem
/
Extraction liquide-liquide
/
Médecine légale
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Journal of Forensic Medicine
Année:
2015
Type:
Article
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