Effects of HO-1 on Lipopolysaccharide-induced Endoplasmic Reticulum Stress of Rat Hepatocytes / 法医学杂志
Journal of Forensic Medicine
;
(6): 417-421, 2015.
Article
Dans Chinois
| WPRIM
| ID: wpr-984019
ABSTRACT
OBJECTIVE@#To investigate effects of antioxidant stress protein heme oxygenase-1 (HO-1) on lipopolysaccharide (LPS)-induced endoplasmic reticulum stress (ERS) of rat hepatocytes.@*METHODS@#The BRL cells (rat hepatocyte cell line) were cultured. The hepatocytes were treated with LPS, LPS+HO-1 siRNA, HO-1 siRNA and PBS solution, respectively. The cell viability was measured by trypan blue exclusion test. The apoptosis cells were detected by the fluorescent dye Hoechst 33258. Expressions of GRP78, CHOP, caspase-12 and HO-1 were detected by Western blotting.@*RESULTS@#LPS caused an increase of HO-1 protein expression of rat hepatocytes in a dose-dependent and time-dependent manner, a up-regulation of GRP78, CHOP and caspase-12, a decrease in cell viability, and an increase in apoptosis rate of hepatocytes. Pretreatment of HO-1 siRNA inhibited the up-regulation of LPS-induced HO-1, however, aggravated ERS and cellular injury.@*CONCLUSION@#HO-1 inhibites ERS-mediated cellular injury of rat hepatocytes induced by LPS.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Lipopolysaccharides
/
Apoptose
/
Hépatocytes
/
Réticulum endoplasmique
/
Heme oxygenase-1
/
Stress du réticulum endoplasmique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Journal of Forensic Medicine
Année:
2015
Type:
Article
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