Changes of AP-1 Binding Activities in Rat Brain by Electroconvulsive Shock / 신경정신의학
Journal of Korean Neuropsychiatric Association
;
: 544-552, 2003.
Article
Dans Coréen
| WPRIM
| ID: wpr-9869
ABSTRACT
OBJECTIVES:
ECS could have therapeutic effects on psychiatric illnesses by inducing IEGs, which in turn regulates expression of their target genes. We observed AP-1 binding activity and identified AP-1 binding proteins in NMDAR1, late response gene of IEGs, which considered as the candidate gene for schizophrenia.METHODS:
By gel shift assay and supershift assay, we observed binding activities and AP-1 binding proteins in NMDAR1. Because IEGs are induced rapidly but transiently by external stimuli, there is a possibility that the expression of IEGs is negatively feedbacked by their own products via their AP-1 binding sites. For that purpose, we also observed AP-1 binding activity of c-fos and c-jun via gel shift and supershift assay.RESULTS:
ECS increased AP-1 binding activities of NMDAR1 gene, contributed by c-Fos and its related proteins. Peak of the increased binding was 60 minutes in both hippocampus and cerebellum. Though expression of c-Fos and c-Jun were increased by ECS, there were no changes in AP-1 binding activities after ECS. AP-1 sites of IEGs were binded by CREB, regardless of ECS.CONCLUSION:
There is a possibility that ECS induced IEG expression, and then incresed expression of NMDR1 by binding of expressed IEGs to the AP-1 site of NMDAR1. ECS did not increase AP-1 binding activities of IEGs. This suggests that the regulation of IEGs' expression can not be influenced mainly by AP-1 site.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Schizophrénie
/
Sites de fixation
/
Encéphale
/
Protéines de transport
/
Cervelet
/
Facteur de transcription AP-1
/
Électrochoc
/
Hippocampe
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Coréen
Texte intégral:
Journal of Korean Neuropsychiatric Association
Année:
2003
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS