Increased Expression of ICAM-1 and VCAM-1 in Human Lupus Nephritis / 대한신장학회잡지

ABSTRACT

Intercellular adhsion molecule-1(ICAM-1) and vascular cell adhension molecule-1(VCAM-1) are important cell-surface glycoprotein regulating interactions among immune cells, such as accessory and T cells. Recently, the cDNA encoding the ICAM-1 and VCAM-1 molecule has been isolated and monoclonal antibodies for ICAM-1 and VCAM-1 have been identified, thus allowing studies of ICAM-1 and VCAM- 1 expression using frozen or paraffin-embedded tissues. To determine whether altered expression of ICAM-1 and VCAM-1 occurs in normal and autoimmune lupus nephritis, we studied the ICAM-1 and VCAM-1 expression in kidneys of five normal human kidney specimens and 39 paraffin-embedded tissues from patients with lupus nephritis. By immunohistochemical staining of our materials, ICAM-1 was expressed in the glomerular cells in all cases with lupus nephritis. It was only expressed in the interstitial cell in one case with normal kidney. VCAM-1 expression was significantly increased in the distal tubules and in the interstitial cells, glomerular endothelial, parietal epithlial cells and urinary spaces with lupus nephritis. It was not expressed in the normal kidney. ICAM-1 and VCAM-1 were strongly expressed in the crescents. These results suggest that ICAM-1 and VCAM-1 play an important role in the pathogenesis of glomerular damage and crescent formation in lupus nephritis.