Detection of Malignant Cells in Pleural Fluid or Ascites by CD44v8-10/CD44v10 competitive RT-PCR
The Korean Journal of Internal Medicine
;
: 30-35, 2001.
Article
Dans Anglais
| WPRIM
| ID: wpr-99478
ABSTRACT
BACKGROUND:
CD44 is a cell surface adhesion molecule which has been implicated in various biologic functions as lymphocyte homing and activation, cellular migration and extracellular matrix adhesion. Over-expression of CD44v8- 10 has been found in several cancers and is considered to be associated with tumor progression and metastasis. Recently, a novel molecular method, CD44v8- 10/CD44v10 competitive reverse transcription-polymerase chain reaction(RT-PCR) has been developed for detecting cancer cells over-expressing CD44v8-10.METHODS:
We analyzed from benign and malignant pleural effusion and ascites by CD44 competitive RT-PCR and compared to the conventional cytology.RESULTS:
The CD44 competitive RT-PCR analysis showed that all the 24 samples associated with benign disease presented a predominant expression of the CD44v10 transcript (v8-10/v10 ratio 0.126-0.948), whereas 6 of 7 malignant pleural samples associated with cytology positive cancer expressed the CD44v8-10 transcript (v8-10/v10 ratio > 1.00).CONCLUSION:
These results indicate that CD44 competitive RT-PCR assay is a useful and adjunct to cytological examination in cancer diagnosis, especially in detecting exfoliated cancer cells in pleural effusion.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Ascites
/
Étude comparative
/
Données de séquences moléculaires
/
Séquence nucléotidique
/
Épanchement pleural malin
/
Sensibilité et spécificité
/
Carcinome pulmonaire non à petites cellules
/
Antigènes CD44
/
RT-PCR
/
Tumeurs gastro-intestinales
Type d'étude:
Etude diagnostique
Limites du sujet:
Adulte
/
Adulte très âgé
/
Aged80
/
Femelle
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
The Korean Journal of Internal Medicine
Année:
2001
Type:
Article
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