Hydrogen sulfide protects H9c2 cardiomyoblasts against H2O2-induced apoptosis
Braz. j. med. biol. res
;
52(4): e7626, 2019. graf
Artigo
em Inglês
| LILACS
| ID: biblio-1001516
ABSTRACT
Reactive oxygen species (ROS) are highly reactive chemical species that may cause irreversible tissue damage, and play a critical role in cardiovascular diseases. Hydrogen sulfide (H2S) is a gasotransmitter that acts as a ROS scavenger with cardio-protective effects. In this study, we investigated the cytoprotective effect of H2S against H2O2-induced apoptosis in cardiomyocytes. H9c2 rat cardiomyoblasts were treated with H2S (100 μM) 24 h before challenging with H2O2 (100 μM). Apoptosis was then assessed by annexin V and PI, and mitochondrial membrane potential was measured using a fluorescent probe, JC-1. Our results revealed that H2S improved cell viability, reduced the apoptotic rate, and preserved mitochondrial membrane potential. An increased Bcl-2 to Bax ratio was also seen in myocytes treated with H2S after H2O2-induced stress. Our findings indicated a therapeutic potential for H2S in preventing myocyte death following ischemia/reperfusion.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Apoptose
/
Mioblastos Cardíacos
/
Peróxido de Hidrogênio
/
Antioxidantes
Tipo de estudo:
Estudos de avaliação
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
2019
Tipo de documento:
Artigo
País de afiliação:
China
Instituição/País de afiliação:
Institute of Clinical Medicine, Renmin Hospital, Hubei University of Medicine/CN
Similares
MEDLINE
...
LILACS
LIS