An update on the tools for creating transgenic animal models of human diseases - focus on atherosclerosis
Braz. j. med. biol. res
;
52(5): e8108, 2019. tab
Artigo
em Inglês
| LILACS
| ID: biblio-1001521
ABSTRACT
Animal models of diseases are invaluable tools of modern medicine. More than forty years have passed since the first successful experiments and the spectrum of available models, as well as the list of methods for creating them, have expanded dramatically. The major step forward in creating specific disease models was the development of gene editing techniques, which allowed for targeted modification of the animal's genome. In this review, we discuss the available tools for creating transgenic animal models, such as transgenesis methods, recombinases, and nucleases, including zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), and CRISPR/Cas9 systems. We then focus specifically on the models of atherosclerosis, especially mouse models that greatly contributed to improving our understanding of the disease pathogenesis and we outline their characteristics and limitations.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Animais Geneticamente Modificados
/
Engenharia Genética
/
Modelos Animais de Doenças
/
Aterosclerose
/
Nucleases dos Efetores Semelhantes a Ativadores de Transcrição
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
2019
Tipo de documento:
Artigo
País de afiliação:
Federação Russa
Instituição/País de afiliação:
Biocad Biotechnology Company/RU
/
Russian Academy of Sciences/RU
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