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Pancreatic ß-cell function is inhibited by miR-3666 in type 2 diabetes mellitus by targeting adiponectin
Tan, J; Tong, A; Xu, Y.
  • Tan, J; Linyi Central Hospital. Department of Endocrinology. Linyi. CN
  • Tong, A; Linyi Central Hospital. Department of Endocrinology. Linyi. CN
  • Xu, Y; Linyi Central Hospital. Department of Neurology. Linyi. CN
Braz. j. med. biol. res ; 52(6): e8344, 2019. graf
Artigo em Inglês | LILACS | ID: biblio-1001533
ABSTRACT
Type 2 diabetes mellitus (T2D) is a common endocrine and metabolic disorder, and poses threats to human health worldwide. Recently, microRNAs (miRNAs) have been suggested to play important roles in the pathophysiology of T2D. In this study, we explored the role of miR-3666 in T2D. miR-3666 was significantly down-regulated in the serum of T2D patients when compared to that of healthy volunteers, and miR-3666 expression level was negatively correlated with blood glucose levels of T2D patients. Overexpression of miR-3666 inhibited cell proliferation, reduced insulin secretion, and promoted cell apoptosis of pancreatic β-cell line (INS-1 cells). On the other hand, knockdown of miR-3666 had the opposite effects in INS-1 cells. The bio-informatics analysis using TargetScan revealed that adiponectin (ADIPOQ) was a downstream target of miR-3666, and the interaction between miR-3666 and ADIPOQ was validated by luciferase reporter assay. In addition, miR-3666 negatively regulated the mRNA and protein expression of ADIPOQ. Overexpression of ADIPOQ promoted insulin secretion after glucose stimulation, promoted cell proliferation, inhibited cell apoptosis, and partially abolished the effects of miR-3666 overexpression on insulin secretion, cell proliferation, and cell apoptosis of INS-1 cells. In conclusion, our results revealed that miR-3666 inhibited pancreatic cell proliferation, reduced insulin sensitivity, and promoted apoptosis by targeting ADIPOQ.
Assuntos


Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Resistência à Insulina / MicroRNAs / Diabetes Mellitus Tipo 2 / Células Secretoras de Insulina Limite: Feminino / Humanos / Masculino Idioma: Inglês Revista: Braz. j. med. biol. res Assunto da revista: Biologia / Medicina Ano de publicação: 2019 Tipo de documento: Artigo País de afiliação: China Instituição/País de afiliação: Linyi Central Hospital/CN

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Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Resistência à Insulina / MicroRNAs / Diabetes Mellitus Tipo 2 / Células Secretoras de Insulina Limite: Feminino / Humanos / Masculino Idioma: Inglês Revista: Braz. j. med. biol. res Assunto da revista: Biologia / Medicina Ano de publicação: 2019 Tipo de documento: Artigo País de afiliação: China Instituição/País de afiliação: Linyi Central Hospital/CN