Effect of miR-125b on dermal papilla cells of goat secondary hair follicle
Electron. j. biotechnol
;
25: 64-69, ene. 2017. tab, graf, ilus
Artigo
em Inglês
| LILACS
| ID: biblio-1008601
ABSTRACT
Background:
MicroRNAs (miRNAs) are endogenous noncoding RNAs that regulate various biological processes. miR-125b is a miRNA that has been reported to be critical for hair follicle (HF) morphogenesis and development. We identified that the expression of miR-125b varies during an individual hair cycle (anagen, catagen, and telogen) in the skin of cashmere goats. We constructed a gain model (by overexpressing miR-125b) and a loss model (by inhibiting endogenous miR-125b) based on dermal papilla cells (DPCs) to further investigate the role of miR-125b in HF cycle. In addition, we used a dual-luciferase system to highlight the predicated target genes of miR-125b.Results:
We found that miR-125b affects the expression of FGF5, IGF-1, SHH, TNF-α, MSX2, LEF-1, FGF7, NOGGIN, BMP2, BMP4, TGF-ß1, and ß-catenin. The dual-luciferase assay further validated a direct interaction between miR-125b and FGF5 and TNF-α.Conclusion:
miR-125b affects the expression levels of genes related to hair cycle and may also play a critical role in regulating the periodic development of HF.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Folículo Piloso
/
MicroRNAs
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2017
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
China
Instituição/País de afiliação:
Northwest A&F University/CN
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