Highly efficient enzymatic preparation of isomalto-oligosaccharides from starch using an enzyme cocktail
Electron. j. biotechnol
;
26: 46-51, Mar. 2017. graf, tab
Artigo
em Inglês
| LILACS
| ID: biblio-1009650
ABSTRACT
Background: Current commercial production of isomalto-oligosaccharides (IMOs) commonly involves a lengthy multistage process with low yields. Results: To improve the process efficiency for production of IMOs, we developed a simple and efficient method by using enzyme cocktails composed of the recombinant Bacillus naganoensis pullulanase produced by Bacillus licheniformis, α-amylase from Bacillus amyloliquefaciens, barley bran ß-amylase, and α-transglucosidase from Aspergillus niger to perform simultaneous saccharification and transglycosylation to process the liquefied starch. After 13 h of reacting time, 49.09% IMOs (calculated from the total amount of isomaltose, isomaltotriose, and panose) were produced. Conclusions: Our method of using an enzyme cocktail for the efficient production of IMOs offers an attractive alternative to the process presently in use.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Oligossacarídeos
/
Amido
/
Enzimas
/
Isomaltose
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2017
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
China
/
África do Sul
Instituição/País de afiliação:
Durban University of Technology/ZA
/
Fuzhou University/CN
/
Tianjin University of Science and Technology/CN
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