Inter-laboratory ring trial to evaluate real-time reverse transcription polymerase chain reaction methods used for detection of infectious pancreatic necrosis virus in Chile
Electron. j. biotechnol
;
28: 20-26, July. 2017. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-1015729
ABSTRACT
Background: Infectious Pancreatic Necrosis Virus (IPNV) is the etiological agent of a highly contagious disease that affects salmonids. In Chile, the second worldwide salmon producer, IPNV causes great economic loss and is one of the most frequently detected pathogens. Due to its high level of persistence and the lack of information about the efficiency of its diagnostic techniques, the National Reference Laboratory (NRL) for IPNV in Chile performed the first inter-laboratory ring trial, to evaluate the sensitivity, specificity and repeatability of the qRT-PCR detection methods used in the country. Results: Results showed 100% in sensitivity and specificity in most of the laboratories. Only three of the twelve participant laboratories presented problems in sensitivity and one in specificity. Problems in specificity (false positives) were most likely caused by cross contamination of the samples, while errors in sensitivity (false negatives) were due to detection problems of the least concentrated viral sample. Regarding repeatability, many of the laboratories presented great dispersion of the results (Ct values) for replicate samples over the three days of the trial. Moreover, large differences in the Ct values for each sample were detected among all the laboratories. Conclusions: Overall, the ring trial showed high values of sensitivity and specificity, with some problems of repeatability and inter-laboratory variability. This last issue needs to be addressed in order to allow harmonized diagnostic of IPNV within the country. We recommend the use of the NRL methods as validated and reliable qRT-PCR protocols for the detection of IPNV.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Salmonidae
/
Vírus da Necrose Pancreática Infecciosa
/
Infecções por Birnaviridae
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Reação em Cadeia da Polimerase em Tempo Real
/
Doenças dos Peixes
Tipo de estudo:
Estudo diagnóstico
/
Guia de Prática Clínica
Limite:
Animais
País/Região como assunto:
América do Sul
/
Chile
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2017
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Chile
Instituição/País de afiliação:
Universidad de Chile/CL
/
Universidad de Valparaíso/CL
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