Detencción por PCR del ADN de Trypanosoma cruzi con los pares de primers TC-4 y TC-22 / Annual Reports 1991 / Detection of Trypanosoma cruzi DNA with TC-4 and TC-22 primer pairs by PCR
Asunción; EFACIM; dic; 1991. 156-163 p. ilus.
Monography
em Es
| LILACS, BDNPAR
| ID: biblio-1017977
Biblioteca responsável:
PY2.1
Localização: PY3.1 SR 616.9363 An78a 1991
ABSTRACT
chromosomal DNA from a Paraguayan Trypanosoma cruzi isolate was extracted and sonicated. The fragments were cloned and some clones were size-selectd and sequenced. Two primer pairs TC22 and TC4 were synthesized and applied to other Paraguayan T. cruzi isolates and two Leishmania mexicana amazoniensis strains by the polymerase chain reaction. The specificity and sensitivity of these primers were correlated with TCZ primer pair synthesized by Moser et al. Remarkable differences were observed between the three primer pair which could be possibly explained by the fact that TCZ primers unlike TC22 and TC4 amplify a highly repettitive sequence of chromosomal T. cruzi DNA
Buscar no Google
Índice:
LILACS
Assunto principal:
Trypanosoma cruzi
/
Reação em Cadeia da Polimerase
/
Doença de Chagas
Tipo de estudo:
Diagnostic_studies
País/Região como assunto:
America do sul
/
Paraguay
Idioma:
Es
Ano de publicação:
1991
Tipo de documento:
Monography